Commonly described chemical reactions used for bioconjugation include: • Thioether coupling: A reaction between cysteine-free thiols or sulfur-modified lysines on the POI and activated thio-reactive maleimide handles on the linker (or linker+ligand). • Amide coupling: A reaction between lysine-free amines on the POI and … See more The ligand is a synthetic molecule (PAMP) that is recognized by a specific PRRand triggers (agonist) or blocks (antagonist) inflammatory responses. It features a reaction handle (e.g. free amine, carboxylic acid, azido … See more POIs(e.g. monoclonal antibodies, antigenic peptides/proteins) must feature solvent-accessible amino-acidic conjugation sites, cysteines and lysines … See more The linkeris a chemical moiety that covalently bridges the ligand and POI. Linkers can be categorized as non-cleavable or cleavable (for a localized release of the ligand). They can also feature a single or two … See more Web6600 Kenilworth Avenue Riverdale, MD 20737 Phone: 301-699-2255 TTY: 301-699-2544 Email Us
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