http://ghanamedj.org/articles/June2012/Microbial%20Contamination%20in%20Herbal%20final.pdf WebDC3000 genomic DNA (~1000 ng/µl) prepared using the GENTRA Puregene Yeast/Bact. Kit (Qiagen, Valencia CA). 5. Oligonucleotides (10 pmol/µl). flank A forward, flank A reverse . flank B forward, flank B reverse . A seq forward, B seq reverse . ... Gel DNA Recovery Kit and Clean-up and Concentrator Kit-5 (Zymo Research, Orange, CA). 14. Sterile ...
Gentra Puregene Handbook - Emmes
WebOct 6, 2024 · Genomic DNA was extracted from 3-ml overnight cultures using a Gentra Puregene Yeast/Bact Kit (Qiagen, Hilden, Germany). Bacteria were treated with lysis buffer containing Proteinase K and RNaseA for 2 h at 65°C, and DNA purification was performed according to the manufacturer’s recommended protocols. Genome Sequencing and … WebOct 17, 2024 · Gentra Puregene Yeast/Bact. kit (Qiagen) or preferred genomic DNA extraction kit. 6. 4 mg/ml RNase A (Qiagen or equivalent). 7. Covaris microTUBEs: microTUBE-15AFA beads screw-cap vials and M220 holder XTU insert microTUBE 15 μl. For 50 μl sample volumes, use microTUBE-50AFA fiber screw-cap vials and M220 … how to extend auto lock on iphone 13
Isolating DNA: A Comparison Between DNeasy Blood & Tissue Kit …
WebThe Qiagen protocol for purification of genomic DNA from gram-positive bacterial cultures using Yeast/Bact. Kit ( Gentra Puregene Handbook - QIAGEN) was modified to aid in the i... WebGentra Puregene Yeast/Bact. Kit (200 ml) Gentra Puregene Buccal Cell Kit (100) Lytic Enzyme Solution (650 µl) Generation DNA Purif. Solution (1000 ml) Generation DNA Elution Solution (500 ml) QIAseq cfDNA All-in-One Kit (24) QIAseq cfDNA All-in-One Kit (96) WebGentra Puregene Yeast/Bact kits (QIAGEN) were used to purify DNA from cells in late log-phase. Genomic DNA libraries were prepared using the Nextera DNA Sample Preparation Kit (Illumina) with some modifications to the standard protocol to increase throughput, including custom barcodes, smaller reaction sizes, and additional size-selection steps ... how to extend available time on teams